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C or Do is the first note and semitone of the C major scale, the third note of the A minor scale (the relative minor of C major), and the fourth note (G, A, B, C) of the Guidonian hand, commonly pitched around 261.63 Hz. The actual frequency has depended on historical pitch standards, and for transposing instruments a distinction is made between written and sounding or concert pitch. It has enharmonic equivalents of B♯ and D.
In English the term Do is used interchangeably with C only by adherents of fixed Do solfège; in the movable Do system Do refers to the tonic of the prevailing key.